Welcome to Inverted Fluorescence Microscope internet store where we provide good high quality fluorescence microscopes at discount prices.  The inverted fluorescence microscope is the combination of an epi-fluorescence microscope and an inverted tissue culture microscope.  Some living tissue culture specimens are desired to be viewed under fluorescence microscopy. 

In these cases, the inverted fluorescence microscope is the instrument of choice for the microbiologist.  These types of microscopes are often used for medical research into the effectiveness of drug therapies.  Living cells will fluoresce differently than dead biological cells, and cell counts can be done with measuring grids or microscope software when used in conjunction with a digital microscope camera.  With the inverted design, the images are facing upward so they look into the bottom of the petri dish on the microscope stage. 

The living tissue culture specimen is contained within the petri dish and the bottom part is viewed.  The epi fluorescence part of the microscope consists of a high pressure mercury arc bulb as the illumination source.  This light is passed through the dichoric fluorescence filter cube.  The cube has a filter allowing only certain excitation wavelengths to pass to reach the specimen.  Light that reaches the specimen excites molecules and causes them to fluoresce.  It is this emitted fluorescence that is picked up and imaged throught the inverted fluorescence microscope.  Emission filters cut out all but the desired wavelengths to view. 

The type of fluorescence specimen, or the fluorescing dye used determines the type of excitation filter and emission filter to use.  Filter cubes are easily interchangeable, and most inverted fluorescence microscope sets will contain several of varying wavelengths.  Custom made filters are always an option and can be tailored to the specific wavelength requirements of the specimen, thus increasing optical intensity and quality of the viewed image.  If you have a need for an inverted fluorescence microscope, please contact our skilled microscopists today for a recommendation on the best equipment and accessories to meet your needs.